Purification of the protein agglutinating factors in boar seminal plasma

Proteomics of boar seminal plasma

Studies on the Egg Yolk Agglutinating Factors in Boar Semen

The author and his co-workers have found out that, when the diluents including egg yolk for freezing of boar spermatozoa were used, egg yolk agglutinated immediately after the dilution and most of the spermatozoa were enclosed into the agglutinated masses and their motility was drastically suppressed. On the other hand, by using skim milk-diluent widely used for storing liquid boar semen at 15°...

Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody.

For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and...

The Effect of Cattle Seminal Plasma Crude Protein on the Cryopreservation of Goat Semen

This study aimed to improve the quality of cryopreserved goat semen for artificial insemination by the addition of cattle seminal plasma crude protein. Semen collected from cattle and centrifuged to obtain seminal plasma. Then it was purified to obtain the crude protein. The diluent used in this study was milk-yolk. There were three groups, control group without crude protein (P0), the addition...

Protein analysis of boar seminal plasma proteins with protective effect during low-temperature storage of spermatozoa

The present study aimed to investigate the effect of different seminal plasma proteins (SPPs) on boar spermatozoa functional characteristics. We investigated the putative protective effect of SSPs on sperm cells motility and velocity, as well as on the integrity of the plasma membrane (PM) during low-temperature storage at 4 °C. SPPs fractions were obtained and purified by gel permeation chroma...